Даниил Федоров

The effects of severe hypobaric hypoxia and inhibition of hypoxia-inducible factor-1 (hif-1) on biomarkers of cardiac and skeletal muscle injury in rats

• Authors Details :  
• D. A. Fedorov,  
• M. Yu. Frolova,  
• I. E. Krasovskaya,  
• N. V. Kuleva

Journal title : Biophysics

Publisher : Pleiades Publishing Ltd

Online ISSN : 1555-6654

Page Number : 808-811

Journal volume : 64

Journal issue : 5

598 Views Original Article

The goal of the present study was to investigate the molecular mechanisms that underlie heart andskeletal muscle damage in male Wistar rats weighing 200–250 g in response to a 3-h exposure to 180 mm Hg(5% O2) in the model of severe hypobaric hypoxia. It has been demonstrated that the level of the cardiac bio-marker troponin I in the blood plasma of rats exposed to severe hypobaric hypoxia for 3 h increased signifi-cantly compared to the control group, indicating myocardial injury. At the same time, the administration ofthe HIF-1α transcription factor inhibitor did not affect the plasma level of troponin I. In contrast, the releaseof the non-specific biomarker myoglobin into the bloodstream did not increase in response to hypoxia com-pared to the control animals. In addition, 24 h after the exposure to severe hypobaric hypoxia the serum myo-globin level was significantly lower in animals administered with the HIF-1α inhibitor topotecan than in ratsthat did not receive topotecan. Therefore, it may be assumed that the inhibition of the HIF-1α transcriptionfactor 10 min before exposure to severe hypobaric hypoxia reduces skeletal muscle damage. The mechanismsthat affect the adaptation of heart and skeletal muscles to hypoxia are discussed.

Article DOI & Crossmark Data

DOI : https://doi.org/10.1134/S000635091905004X

Article Subject Details

• Biochemistry clinical laboratory
• Biological Sciences
• Molecular biology

Article Keywords Details

• hypoxia-inducible factor HIF-1α
• myocardium
• myoglobin
• topotecan
• troponin I
• Hypoxia

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Article References

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