Biotechnology articles list

“Phyllanthus emblica” known to be amla has role in the skin aging influences the changes in skin, including skin dryness, wrinkle, and irregular pigmentation. Initially the 6 day observation has been taken for the ageing activity to be track to study the TREM2 pathway of “Phyllanthus emblica”. Cellular observation and pathway consideration: The environmental impact of pH, Temperature, Humidity and stability of amla fruits is important for the ageing of cells in neuronal cascade of TREM2 Pathway, while studying the fruits cell cycle. The melanin suppression through inhibition of tyrosinase and tyrosinase-related protein-2 activities, the strong antioxidant, and the potent matrix metalloproteinase-2 in cellular observation of tyrosinase pathway. The study aimed to evaluate the anti-skin aging efficacy of amla.

Wahul UMESH

The screening of perfect diuretics for non-clinical utility in Diabetes mellitus is a relatively novel approach which gain inssight into underlying the pathophysiological processes. This study is aims to evaluate the diuretic effect of a crude aqueous & alcoholic extract of M.charantia Linn. using Albino Wistar Rat model. In this study, the comparative observation of Diuretic activity with standard and extracted compound were shown that the estimated 24‐hour urine contains the Na+ - 3.82 g, 3.82 g, 3.92 g and K+ - 1.35 g, 1.39 g, 1.48 g wt. for Vehicle control, Standard drug, and Extracted compound respectively. Which possess the favoring result means from the spot urine were 10.7±7.0 g/24 h and 3.9±2.1 g/24 h, respectively. Coefficients were 0.035, 0.022, 0.046 at (d±2SD = 7.07 g, 4.42 g and 8.92 g) for sodium chloride and 0.068, 0.031, 0.046 at (d±2SD =4.92 g, 2.31 g, and 3.34 g) for potassium chloride. The Na+ and K+ results can be deduced by conversion (1 g NaCl=0.4 g Na+, 1 g KCl≈0.5 g K+). The present study guide formulation of non clinical trials with statistical study to further measuring the claimed efficacy of M. charantia as a natural remedy for diabetes mellitus.

Wahul UMESH

“Phyllanthus emblica” known to be amla has role in the skin aging influences the changes in skin, including skin dryness, wrinkle, and irregular pigmentation. Initially the 6 day observation has been taken for the ageing activity to be track to study the TREM2 pathway of “Phyllanthus emblica”. Cellular observation and pathway consideration: The environmental impact of pH, Temperature, Humidity and stability of amla fruits is important for the ageing of cells in neuronal cascade of TREM2 Pathway, while studying the fruits cell cycle. The melanin suppression through inhibition of tyrosinase and tyrosinase-related protein-2 activities, the strong antioxidant, and the potent matrix metalloproteinase-2 in cellular observation of tyrosinase pathway. The study aimed to evaluate the anti-skin aging efficacy of amla.

Dr. Wahul Umesh B

The screening of perfect diuretics for non-clinical utility in Diabetes mellitus is a relatively novel approach which gain inssight into underlying the pathophysiological processes. This study is aims to evaluate the diuretic effect of a crude aqueous & alcoholic extract of M.charantia Linn. using Albino Wistar Rat model. In this study, the comparative observation of Diuretic activity with standard and extracted compound were shown that the estimated 24‐hour urine contains the Na+ - 3.82 g, 3.82 g, 3.92 g and K+ - 1.35 g, 1.39 g, 1.48 g wt. for Vehicle control, Standard drug, and Extracted compound respectively. Which possess the favoring result means from the spot urine were 10.7±7.0 g/24 h and 3.9±2.1 g/24 h, respectively. Coefficients were 0.035, 0.022, 0.046 at (d±2SD = 7.07 g, 4.42 g and 8.92 g) for sodium chloride and 0.068, 0.031, 0.046 at (d±2SD =4.92 g, 2.31 g, and 3.34 g) for potassium chloride. The Na+ and K+ results can be deduced by conversion (1 g NaCl=0.4 g Na+, 1 g KCl≈0.5 g K+). The present study guide formulation of non clinical trials with statistical study to further measuring the claimed efficacy of M. charantia as a natural remedy for diabetes mellitus.

Dr. Wahul Umesh B

The study of diabetes is not only limited to particular symptoms, but it is consequently affects the pathological and functional changes in the metabolic pathways of human body system. In those symptomatic diseases various drugs are used to treat the diabetes such as biosimilar therapy including use of insulin and insulin analogues, oral hypoglycaemic agents and various other complementary medicines. In understanding of suggested potential antidiabetic, effect of M. charaantia Linn. on fasting blood sugar levels and its biochemical analysis in alloxan- induced diabetic rats were investigated. The extracts of M. charaantia Linn. Produced a significant antidiabetic activity at normal dose levels of their lethal doses. A comparison between the action of reduction in blood glucose level in different dose forms of M. charantia extract and Std. drug were seen. An oral glucose tolerance or oral tolerance test were performed with the use of glucose strip Accu-check meter. The different extract viz. ethanol extract + water, petroleum ether + Isopropyl alcohol extract were used for further dosing purpose. The ethanol + water extract were showed significant (P<0.001) antidiabetic activity. In alloxan induced rat model blood glucose level were as, 214.5±5 mg/dLfor std.drug and 216.5±5 mg/dL in comparison with diabetic control 225.5±5 mg/dL. An ANOVA was used for the statistical analysis and p-values less than 0.01 compared to normal group and 0.05 compared to diabetic control group were considered statistically significant. The extract of M.charantia Linn. from seed at the dose of 250 mg/kg, significantly shows the better result in reduction of blood glucose level as compared to the concentration of 500 mg/kg. The increased level of glucose due to the damage of pancreas showed regeneration of pancreatic enzymes by extract of M. charaantia Linn. Which were damaged by alloxan treatment. These solvent extract also balance the body weight loss in diabetic rat, hence the present extract shows the potential to act as antidiabetic drug.

Dr. Wahul Umesh B

As we aware diabetes is not only one kind of symptomatic disease but its occurrence spread through the various metabolic channels and hence raises other disorders. The prolonged symptoms of diabetes also cause the complications of eyesight, Night blindness, kidney failure, and other autoimmuno dysfunction including sexual dysfunction. In those symptomatic diseases various drugs are used to treat the diabetes such as biosimilar therapy including use of insulin and insulin analogues, oral hypoglycaemic agents and various other complementary medicines. As herbal remedies i.e. M charantia Linn. (Bitter Gourd) are commonly known as fruit vegetables. The Leaves, Seeds, Roots, Fruits and the stem part of the plants are medicinally used in different diseases. It is most effectively used to treat the acidic condition of gastrointestinal tract. M.charantia is also called the oxygen radical scavenger, which takes part into metabolic pathway. Due to the oxygen radical scavenging activity of GSH it directly expedites the ROS neutralization and the repair of ROS-induced damage which is important to neutralize the acidic condition of gastrointestinal tract.The present investigation was carried out to study the characterization of present antidiabetic compound having different solvent extract of M.charantia in various solvent system. The overall conclusion suggested that the extracted compound shows the antidiabetic and diuretic properties. The total unknown protein concentration was 21.01 µg/mL which is similar with standard antidiabetic drug and the slope consists of 0.0314 with the line of intercept 0.081, which has been elaborated in results and conclusion.

Dr. Wahul Umesh B

There are several significant assays including bioavailability, Toxicity, high clearance, and further current therapies require higher and frequent dosing to gain desired therapeutic effects for Rheumatoid arthritis (RA). Nanoparticles have beenwidely investigated for rheumatoid arthritis (RA). Though, higher doses of Nanocarriers also increase the incidence of dreadful adverse effects. Wherever, nanocarrier properties are most populated by the use of different approaches like targeting ligands, to change the physicochemical properties including higher encapsulation, better penetrating ability in cells etc. The main advantage of the nanocarrier that they provide longer circulation which enhances drug localization at the inflamed site and selective targeting to enhance the therapeutic index of anti-arthritic drugs. However, the optimal properties of nanocarrier also depend on the route of administration and size of particles, thus larger size show more retention upon local injection and smaller sized ones are more optimal for passive targeting. The present article discusses the emergence of nano-carriers for antirheumatic drugs, which delivers drug molecule to the inflamed site by topical, intra-articular (i.a) and intra-venous (i.v) administration to take advantage of therapeutic efficacy by passive and active drug delivery. Advancements have been made extensively but still better investigations are needed to achieve the risk-benefit ratio for the development of safe, stable and effective targeting nanocarriers for the e treatment of rheumatoid arthritis (RA).

Dr. Wahul Umesh B