Karishma

K₂edta versus k₃edta stability in yemeni laboratories: toward climate-adapted protocols for resource-limited settings

• Authors Details :  
• Naif Taleb Ali

Journal title : Yemen Journal of Medicine

Publisher : Mansa STM Publishers

Online ISSN : 2583-4614

Page Number : 372-378

Journal volume : 04

Journal issue : 02

27 Views Original Article

Background: Preanalytical variability due to anticoagulant choice remains inadequately characterized in resource-limited tropical settings like Yemen, where environmental stressors exacerbate challenges. This study aimed to compare the stability and cost-effectiveness of K₂EDTA and K₃EDTA anticoagulants for complete blood count testing under simulated Yemeni conditions. Methods: Prospective study of 100 healthy adult male volunteers in Ad’Dla Governorate, Yemen (January–March 2025). Paired samples in K₂EDTA/K₃EDTA tubes were analyzed at baseline (T0) and after 6-hour storage at 22 ± 2°C (T6). Statistical analysis included Bonferroni-corrected repeated-measures ANOVA and paired t-tests. Results: Fresh samples showed high inter-anticoagulant concordance (ICC > 0.90). After 6-hour storage, K₂EDTA exhibited significant Mean Corpuscular Volume (MCV) reduction (Δ = −2.33 fL, p < 0 xss=removed xss=removed xss=removed> 2 hours), while K₃EDTA is preferable for immediate analysis. These findings provide preliminary guidance for hematology testing in Yemeni laboratories and similar tropical settings, pending validation in diverse populations and extreme conditions.

Article DOI & Crossmark Data

DOI : https://doi.org/10.63475/yjm.v4i2.0150

Article Subject Details

• Clinical pathology
• Hematology
• Medicine

Article Keywords Details

• EDTA anticoagulants
• resource-limited settings
• Sysmex KX-21N
• tropical hematology
• Yemen
• Yemeni laboratories
• Preanalytical variability

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