Biotechnology articles list

Nano technology refers to enhancing the function and delivering the upcoming smart solutions to products at the nanoscale level. It relates to organizing the molecules for altering in dimension and characteristic for improvement like smart fabric. These fabrics could assist producers with the added prominence on a standard of living, visual attraction, and system wanted technological produces. Nanosize particles can unveil unpredicted characteristics dissimilar from the bulk matter. The fundamental principle is that the features could radically be altered after the material is decreased to the nanometer scale. Nanotechnology has multipurpose functions in fabric manufacturing in producing the stain and wrinkles defiance, flame retardant, antimicrobial and antistatic properties, moisture control, ultraviolet protection, and release features. The nanomaterials inside the fabric could influence numerous qualities, comprising reduction, electrical conductivity, flammability, and strength. Nanotechnology has additionally created a significant impression on various application and implementation. Nano-doctored fabrics may advance numerous fabrications as the nano-science progresses further.

Munima Haque

India is a country of immense diversity. It is home to people of many different racial, languages, ethnic, religious, and national backgrounds. Groups of people in India differ from each other not only in physical or demographic characteristics but also in distinctive patterns of behavior and these patterns are determined by social and cultural factors like language, region, religion, and caste. Apart from behaviour, economic development, level of education and political culture of the people in various social segments differ from region to region. More you can say that economy and cultures have been enriched by the contributions of migrants from round the globe. In an increasingly globalised world, migratory movements is continuously shaping the countries all over the world. Some countries like India and Ireland, which set the example of economic development and social integration, have the positive impact of the migration by globalisation and some countries like USA, which recently witness racism, xenophobia and discrimination have the negative impact on the migrants. It does not mean India do not face fragmentation and USA do not have cohesion. USA have many stories which show successful integration process, that facilitated the lives of immigrant communities, but being a developed country it still suffers from cultural alienation. In these countries, borders are built within borders to create cultural divides that do not allow people to integrate. Recently, this problem has become more prominent due to the rise of terrorism, clash of cultures in the world, leading to the glorification of stereotypes. People are becoming less accepting towards anyone who does not belong to their region. Migration does not stop after people move from one place to another place. The main question start after that ‘now what’ they will do. That is why this topic needs to be discussed thoroughly in order to find better solutions. This paper will begin with an analysis of different approaches to Migration, discuss the target groups for integration policies, provide indicators of the current situation of migrants and proceed to an analysis of integration tools: legislation, social policies and participatory processes. It will focus not only on the impact of migration but also on social integration, mix culture like indo-western culture in a comparative basis.

Ekta Meena

India is a country of immense diversity. It is home to people of many different racial, languages, ethnic, religious, and national backgrounds. Groups of people in India differ from each other not only in physical or demographic characteristics but also in distinctive patterns of behavior and these patterns are determined by social and cultural factors like language, region, religion, and caste. Apart from behaviour, economic development, level of education and political culture of the people in various social segments differ from region to region. More you can say that economy and cultures have been enriched by the contributions of migrants from round the globe. In an increasingly globalised world, migratory movements is continuously shaping the countries all over the world. Some countries like India and Ireland, which set the example of economic development and social integration, have the positive impact of the migration by globalisation and some countries like USA, which recently witness racism, xenophobia and discrimination have the negative impact on the migrants. It does not mean India do not face fragmentation and USA do not have cohesion. USA have many stories which show successful integration process, that facilitated the lives of immigrant communities, but being a developed country it still suffers from cultural alienation. In these countries, borders are built within borders to create cultural divides that do not allow people to integrate. Recently, this problem has become more prominent due to the rise of terrorism, clash of cultures in the world, leading to the glorification of stereotypes. People are becoming less accepting towards anyone who does not belong to their region. Migration does not stop after people move from one place to another place. The main question start after that ‘now what’ they will do. That is why this topic needs to be discussed thoroughly in order to find better solutions. This paper will begin with an analysis of different approaches to Migration, discuss the target groups for integration policies, provide indicators of the current situation of migrants and proceed to an analysis of integration tools: legislation, social policies and participatory processes. It will focus not only on the impact of migration but also on social integration, mix culture like indo-western culture in a comparative basis.

Ekta Meena

In this paper, investigations are made to analyze the human body temperature during wound healing process due to surgery. Wound is considered after the skin graft. Skin graft is a technique used in plastic surgery. Skin is the first line of defense between the human and environment, it is very susceptible to damage. Internal body or core temperature (Tb) is one of the clinical vital signs along with pulse and respiratory rates. Any disturbance in body temperature will drive complexities in wound healing process. These studies are important in the mechanism of establishing the limits of thermal regulation of human body during the healing process in different situations and conditions. The Finite element method is used to analyze tissues temperature for normal tissues (donor site) and abnormal tissues (tissues after surgery). Appropriate boundary conditions have been framed. Numerical results are obtained using Crank Nicolson Method.

Manisha Jain

Physically unclonable function (PUF) is a hardware security module preferred for hardware feature based random number and secret key generation. Security of a cryptographic system relies on the quality of the challenge-response pair, it is necessary that the key generation mechanism must unpredictable and its response should constant under different operating condition. Metastable state in CMOS latch is undesirable since it response becomes unpredictable, this feature used in this work to generate a unique response. A feedback mechanism is developed which forces the latch into the metastable region; after metastable state, latch settle to high or state depends on circuit internal condition and noise which cannot be predicted. Obtained inter hamming variation for 8 PUF is 51% and average intra hamming distance is 99.76% with supply voltage variation and 96.22% with temperature variation.

Abhishek Kumar

One of the unique features of Indian society is prevalence of caste system which was originated thousands of years back to demarcate the people engaged in different occupation or jobs. Initially it was not much rigid but gradually people belonging to upper castes for their own selfish means to maintain their monopoly made this arrangement hereditary and started treating people of lower castes disgracefully. For preservation of this system, people started controlling their women to prevent inter-caste marriages and the concept of endogamy came up. This robbed away many types of freedom from women. For women belonging to lower castes, this situation is worse as they are doubly subjugated on the basis on caste as well as gender. Men belonging to their own caste treat them as secondary beings. This paper throws light on this intersection. How intersection of these two kinds of inequalities place them at the lowest position in Indian society. Dr. B.R. Ambedkar rises as their leader who all his life worked for empowerment of downtrodden section of society. He argues that education is the primary tool for evading these differences among people. He further emphasizes to adopt the concept of exogamy to break the backbone of Indian caste system and to immediately leave a religion or culture which legitimizes such system of inequality among people of the same land.

Swati sharma

One of the unique features of Indian society is prevalence of caste system which was originated thousands of years back to demarcate the people engaged in different occupation or jobs. Initially it was not much rigid but gradually people belonging to upper castes for their own selfish means to maintain their monopoly made this arrangement hereditary and started treating people of lower castes disgracefully. For preservation of this system, people started controlling their women to prevent inter-caste marriages and the concept of endogamy came up. This robbed away many types of freedom from women. For women belonging to lower castes, this situation is worse as they are doubly subjugated on the basis on caste as well as gender. Men belonging to their own caste treat them as secondary beings. This paper throws light on this intersection. How intersection of these two kinds of inequalities place them at the lowest position in Indian society. Dr. B.R. Ambedkar rises as their leader who all his life worked for empowerment of downtrodden section of society. He argues that education is the primary tool for evading these differences among people. He further emphasizes to adopt the concept of exogamy to break the backbone of Indian caste system and to immediately leave a religion or culture which legitimizes such system of inequality among people of the same land.

Swati sharma

Here, we report the draft genome sequences of Bacillus subtilis A1, Sphingobacterium sp. strain A3, and Pseudomonas sp. strain A29; Sphingobacterium sp. A3 and Pseudomonas sp. A29 were identified as Bacillus velezensis strain A3 and Bacillus subtilis strain A29, respectively, after a quality control check of the wholegenome sequences deposited in the NCBI database. These bacteria exhibit tremendous production of siderophores and significant antimicrobial potential. When inoculated on maize, these isolates increase its yield

Oluwaseyi Samuel Olanrewaju

Liposomes are a novel drug delivery system (NDDS), they are vesicular structures consisting of bilalyers which form spontaneously when phospholipids are dispersed in water. They are microscopic vesicles in which an aqueous volume is entirely enclosed by a membrane composed of lipid bilayers. NDDS aims to deliver the drug at a rate directed by the needs of the body during the period of treatment and direct the place of action. Liposomes are colloidal spheres of cholesterol non-toxic surfactants, sphingolipids, glycolipids, long chain fatty acids and even membrane proteins and drug molecules or it is also called vesicular system. It differs in size, composition and charge and drug carrier loaded with variety of molecules such as small drug molecules, proteins, nucleotides or plasmids etc. Few drugs are formulated as liposomes to improve their therapeutic index. Hence a number of vesicular drug delivery systems such as liposomes, niosomes, transfersomes and pharmacosomes are developed. The focus of this chapter is to the various method of preparation, characterization of liposomes, advantages and applications, etc. Liposomes for Drug Delivery - liposomes

Kamal Singh

As one of the flow-based passive sorting, the hydrodynamic filtration using a microfluidic-chip has shown to effectively separate into different sizes of subpopulations from cell or particle suspensions. Its model framework involving two-phase Newtonian or generalized Newtonian fluid (GNF) was developed, by performing the complete analysis of laminar flow and complicated networks of main and multiple branch channels. To predict rigorously what occurs in flow fields, we estimated pressure drop, velocity profile, and the ratio of the flow fraction at each branch point, in which the analytical model was validated with numerical flow simulations. As a model fluid of the GNF, polysaccharide solution based on Carreau type was examined. The objective parameters aiming practical channel design include the number of the branches and the length of narrow section of each branch for arbitrary conditions. The flow fraction and the number of branches are distinctly affected by the viscosity ratio between feed and side flows. As the side flow becomes more viscous, the flow fraction increases but the number of branches decreases, which enables a compact chip designed with fewer branches being operated under the same throughput. Hence, our rational design analysis indicates the significance of constitutive properties of each stream.

Myung-Suk Chun

Human bone marrow-derived mesenchymal stem cells (hMSCs) consist of heterogeneous subpopulations with different multipotent properties: small and large cells with high and low multipotency, respectively. Accordingly, sorting out a target subpopulation from the others is very important to increase the effectiveness of cell-based therapy. We performed flow-based sorting of hMSCs by using optimally designed microfluidic chips based on the hydrodynamic filtration (HDF) principle. The chip was designed with the parameters rigorously determined by the complete analysis of laminar flow for flow fraction and complicated networks of main and multi-branched channels for hMSCs sorting into three subpopulations: small (<25>40 μm) cells. By focusing with a proper ratio between main and side flows, cells migrate toward the sidewall due to a virtual boundary of fluid layers and enter the branch channels. This opens the possibility of sorting stem cells rapidly without damage. Over 86% recovery was achieved for each population of cells with complete purity in small cells, but the sorting efficiency of cells is slightly lower than that of rigid model particles, due to the effect of cell deformation. Finally, we confirmed that our method could successfully fractionate the three subpopulations of hMSCs by analyzing the surface marker expressions of cells from each outlet.

Myung-Suk Chun

Therapeutic nucleic acids demand specificity and accuracy in design as well as delivery strategies used in replacement or silencing of the target gene. Gene therapy is believed to be the therapy in which the root cause of the diseases can be treated at the molecular level. Generally gene therapy helps in the identification of the origin of the disorder instead of using drugs to diminish or control the symptoms. The application of nucleic acids to treat and control diseases is known as “gene therapy.” Gene therapy consists on the substitution or addition of a functional gene into the nucleus of a living cell, in order to treat a disease or repair a dysfunction, caused by this gene failure. This therapy is used to correct defective genes, which are responsible for genetic diseases. Thus, gene therapy can be used to prevent, treat or regulate hereditary or acquired disorders, by the production of therapeutic proteins. The gene therapy is mediated by the use of viral and non-viral vectors to transport foreign genes into somatic cells to restorative defective genes. This review focuses on viral vectors in detail.

Dr.P.V.Kamala Kumari

Strain improvement studies were conducted for the production of L-asparaginase from a marine fungus Beauveriabassiana SS18/14 by employing physical and chemical mutagens, in a systemic manner to obtain mutants that have higher L-asparaginase production. The wild strain produced 6.32 IU/mL of L-asparaginase activity while the UV mutant UVF-4 yielded 8.34 IU/mL and nitrous acid mutant UVF4-N-2 exhibited 10.44 IU/mL enzyme activity. The overall strain improvement programme increased L-asparaginase activity 1.65 times with respect to the parent wild strain.

Dr.P.V.Kamala Kumari

The 2019 coronavirus (COVID-19) pandemic is spreading worldwide, with a spectacular increase in death missing any effective therapeutic treatment up to now. Molecular docking is a recognized computational tool to assist in early drug discovery and development. Molecular docking analysis was carried out using 2-(4-(aminomethyl)phenyl)isoindoline-1,3-dione Schiff base conjugates with SARS-CoV-2 protease enzyme and COVID-19 main protease in apo form 6M03). The compounds with the best normalized docking scores to protease enzyme (6LU7) were ARG3 (-8.1 kcal/mole), ARG7 (-8.1 kcal/mole) and ARG6 (-8.0 kcal/mole). The best docking ligands for main protease in apo form (6M03) were ARG7 (-8.7 kcal/mole), ARG6 (-8.6 kcal/mole) and ARG3 (-8.4 kcal/mole). The structural similarity between these conjugates inspired us to perform in silico studies to check their possible binding interactions with essential SARS-CoV-2 proteins. These studies provide insight into the potential binding between Schiff base derivatives and SARS-CoV-2 proteins to provide an insight for finding an effective therapy. Finally, ADMET calculations were performed for the Schiff base compounds to predict their pharmacokinetic profiles.

Mediterranean Journal of Pharmacy and Pharmaceutical Sciences

Strain improvement studies were conducted for the production of L-asparaginase from a marine fungus Beauveriabassiana SS18/14 by employing physical and chemical mutagens, in a systemic manner to obtain mutants that have higher L-asparaginase production. The wild strain produced 6.32 IU/mL of L-asparaginase activity while the UV mutant UVF-4 yielded 8.34 IU/mL and nitrous acid mutant UVF4-N-2 exhibited 10.44 IU/mL enzyme activity. The overall strain improvement programme increased L-asparaginase activity 1.65 times with respect to the parent wild strain

Dr.P.V.Kamala Kumari

Introduction: Obesity has been declared an epidemic that does not discriminate based on age, gender, or ethnicity and thus needs urgent containment and management. Since the third wave of COVID-19 is expected to affect children the most, these children and adolescents should be more cautious while having junk foods, during covid situations due to the compromise of Immunity in the individuals and further exacerbating the organ damage. Methodology: A PAN India survey organized by the Centre for Science and Environment (CSE) among 13,274 children between the ages 9–14 years reported that 93% of the children ate packed food and 68% consumed packaged sweetened beverages more than once a week, and 53% ate these products at least once in a day. Almost 25% of the School going children take ultra-processed food with high levels of sugar, salt, fat, such as pizza and burgers, from fast food outlets more than once a week. Children and adolescents who consume more junk food or addicted to such consumption might be even more vulnerable during the third wave, which will significantly affect the younger category. Conclusion: There is an urgent need to spread awareness among children and young adults about these adverse effects of junk food. There is no better time than now to build a supportive environment nurturing children and young adults in society and promising good health.

Ankul Singh

The result of this study showed that the growth performance of the two varieties of amaranth were greatly reduced, in terms of number of leaves per plant in the saline treated plant exposed to the highest saline concentration compared to the control. Amaranthus cruentus had more number of leaves (6.63) than Amaranthus hybridus (5.67) and Amaranthus hybridus had higher survival rate than Amranthus cruentus(1.83). Although both test plant varieties responded to varying saline concentrations in various ways, both tends to show similar responds. Also, the number of days to germination increases with increased salinity concentrations the two amaranth varieties was both adversely affected by higher saline concentrations, where increased salinity concentrations caused reduction in shoot length Also, the leave breadth was better in the control and the plants with lower amounts of NaCl concentration(0.025 and 0.05), this implies that Amaranthus hybridus and Amaranthus cruenthus tolerate moderate salinity levels, while the plants with higher salinity concentrations (0.075,0.01,0.15) had leave breadth that were small, and eventually died because of high amounts of salt.

Barka PETER

Abstract In the existing investigation, experiments were carried out to assess the F2 population’s resultant of crosses between improved Basmati 370 and MAS25 for various agronomical and root traits cultivated under aerobic water conditions. Large variations for grain yield, root length, root dry biomass, root thickness and length/breadth ratio of grain have been displayed in segregating F2 populations. A close examination of phenotypic correlation exhibited that in the F2 population, root length was certainly matched up (r = 0.496) with root thickness. A DNA fingerprinting catalog for the currently studied F2 generation was arranged using 61 polymorphic SSR markers. Composite Interval Mapping (CIM) inspection with WinQTL cartographer version 2.5 disclosed 13 putative QTL (Quantitative Trait Loci), out of which 6 QTL were for root characters 7 QTL for agronomical characters situated on 1, 2, 3, 8 and 10 chromosomes. The QTL documented in the above-said generation, some promising F2 plants were also scrutinized and found in the homozygous or heterozygous state with high repetitions.

Rajesh Yogi

Plants produce a diverse range of bioactive compounds making them a rich source of different types of medicines. Ornamental plants are cultivated for adornment and to enhance the appearance of houses and also for commercial purposes. However, only very few of these ornamental plant species have found to be used in medicine and only little literature exit on their chemical and biological actions. In the present study, the evaluation of antimicrobial activities and identification of bioactive compounds using TLC and GC-MS of the A. columnaris bark extract were performed. In GC-MS bioactive compounds with medicinal value were identified, such as Benzoic acid, 1H-N-Hydroxynaphth (2,3) imidazole-6,7-dicarboximide, 2-Propenoic acid, 3-(4-methoxyphenyl), 1H-N-Hydroxynaphth (2,3-d) imidazole-6,7-dicarboximi. To prove the nontoxic nature of the plant, its crude bark extract was subjected to toxicity study using human embryonic kidney cell line. It reveal that the plant is minimal toxic to the human kidney cell line so usage of appropriate level will found to be safe and also carrying out some structural modification will help in the extraction of new drugs for pharmaceutical purpose.

Dr. SARANYA DEVI K

Uric acid has increasingly been associated with insulin resistance, hyperinsulinemia, and type 2 diabetes mellitus. Diabetic patients who are hyperuricemic have a risk of developing diabetic complications. Pathogenesis of uric acid may decrease nitric oxide bioavailability in vascular smooth muscle, endothelial cells and direct scavenging of nitric oxide by uric acid. A decrease in endothelial nitric oxide production by uric acid has also been associated with endothelial dysfunction and insulin resistance. This study aims is to determine the relationship between uric acid and glucose levels in patients with type 2 diabetes mellitus. The study included 161 Libyan patients (67 males and 94 females) diagnosed with type 2 diabetes mellitus. Both levels of serum uric acid and hemoglobin A1c (HbA1c) were determined. The patients were divided into two groups. The controllable diabetic group with HbA1c of less than 06.0% and the uncontrollable diabetic group with HbA1c of more than 06.0%. Patients who are suffering from type 2 diabetes mellitus without complications were included whereas patients with smoking, alcoholism, nephrotic disease, malignancy, hepatitis, and renal failure or kidney disease were excluded. The mean and standard deviation of uric acid, HbA1c and Pearson correlation coefficient test were considered. In the controllable diabetic group, serum uric acid mean was found to be 4.807 ± 1.39 and HbA1c was found to be 5.032 ± 1.39. In the uncontrollable diabetic group, serum uric acid was 4.897 ± 1.66 and HbA1c was 8.396 ± 1.65. Uric acid level has significantly been correlated with HBA1C in controlled and uncontrolled diabetic patients (p < 0.05). In addition, the uric acid level was found to be higher in uncontrolled diabetic group than that in the controlled group (p < 0.05). A possible relationship between serum uric acid and incidence of type 2 diabetes mellitus was noted. Thus, uric acid can be used as a potential biomarker to indicate impaired glucose metabolism.

Mediterranean Journal of Pharmacy and Pharmaceutical Sciences